View by:







Application of Cloning to the Production of Biopharmaceuticals to Treat Human and Animal Disease

Eric Overström. Professor, Tufts University, School of Veterinary Medicine

    Health Care: Human & Animals

    Nuclear Transfer – Cloning


    • Proven methodology in several species, recent reports of success in Sheep, cattle & pigs
    • Good efficiency, < ~ 98% of offspring are transgenic, demonstrates stable transgene integration & expression
    • Germline transgenesis
    • Less costly due to transfection/selection in vitro


    • Methodology not optimized in domestic animals
    • Strategies for “knockout” & “knockin” constructs

    Table 1. Development of caprine embryos reconstructed by nuclear transfer using CFF6 transgenic fetal somatic cells

    Nuclear transfer protocols  Metaphase
    II- EtOH
    Oocytes reconstructed  138  92  55
    Oocyte lysis (%)  67 (48.5)  38 (41.3)  23 (41.8)
    Embryo cleavage (%)  48 (34.8)  41 (44.6)  31 (58.4)
    Embryos transferred (%)  37 (34)  38 (41.3)  27 (49.1)
    Recipients  15  14  9
    Ultrasound positive (%)
    30 days 
    9 (60)  11 (78.6)  5 (55.5)
    40 days  1 (6.7) 1 (7.1) 0
    60 days  1 (6.7) 1 (7.1) 0
    Term pregnancy (%) 1 (6.7) 1 (7.1) 0
    Offspring  1 2 0
    % of embryos transferred  2.1 5.2 0
    % of embryos reconstructed 0.7 2.2 0


    Table 2. rhAT expression in CFF6-1 goat milk from an induced lactation

    CFF6-1 induced
    Lactation day 
    hAT concentration
    hAT activity
    Day 3   4.7 N/D
    Day 5  5.8  20.5
    Day 6  5.0  18.3
    Day 7  4.7  15.6
    Day 8  4.1  14.4
    Day 9  3.7  14.6

    N/D: not determined


    • Rational application of animal cloning to the production of biopharmaceuticals
    • Benefit to human and animal health
    • Animal welfare is not compromised
    • Advance fundamental understanding of mechanism that regulate normal and abnormal cell growth

    Email link | Feedback | Contributed by: Boston University. Video adapted from the Issues for the Millennium Workshop

    Topic Sets Available

    AAAS Report on Stem-Cells

    AstroTheology: Religious Reflections on Extraterrestrial Life Forms

    Agency: Human, Robotic and Divine
    Becoming Human: Brain, Mind, Emergence
    Big Bang Cosmology and Theology (GHC)
    Cosmic Questions CD-ROM Preview...
    Cosmic Questions Interviews

    Cosmos and Creator
    Creativity, Spirituality and Computing Technologies
    CTNS Content Home
    Darwin: A Friend to Religion?
    Demystifying Information Technology
    Divine Action (GHC)
    Dreams and Dreaming: Neuroscientific and Religious Visions'
    E. Coli at the No Free Lunchroom
    Engaging Extra-Terrestrial Intelligence: An Adventure in Astro-Ethics
    Evangelical Atheism: a response to Richard Dawkins
    Ecology and Christian Theology
    Evolution: What Should We Teach Our Children in Our Schools?
    Evolution and Providence
    Evolution and Creation Survey
    Evolution and Theology (GHC)
    Evolution, Creation, and Semiotics

    The Expelled Controversy
    Faith and Reason: An Introduction
    Faith in the Future: Religion, Aging, and Healthcare in the 21st Century

    Francisco Ayala on Evolution

    From Christian Passions to Scientific Emotions
    Genetic Engineering and Food

    Genetics and Ethics
    Genetic Technologies - the Radical Revision of Human Existence and the Natural World

    Genomics, Nanotechnology and Robotics
    Getting Mind out of Meat
    God and Creation: Jewish, Christian, and Muslim Perspectives on Big Bang Cosmology
    God, Humanity and the Cosmos: A Textbook in Science and Religion
    God the Spirit - and Natural Science
    Historical Examples of the Science and Religion Debate (GHC)
    History of Creationism
    Intelligent Design Coming Clean

    Issues for the Millennium: Cloning and Genetic Technologies
    Jean Vanier of L'Arche
    Nano-Technology and Nano-ethics
    Natural Science and Christian Theology - A Select Bibliography
    Neuroscience and the Soul
    Outlines of the Science and Religion Debate (GHC)

    Perspectives on Evolution

    Physics and Theology
    Quantum Mechanics and Theology (GHC)
    Questions that Shape Our Future
    Reductionism (GHC)
    Reintroducing Teleology Into Science
    Science and Suffering

    Scientific Perspectives on Divine Action (CTNS/Vatican Series)

    Space Exploration and Positive Stewardship

    Stem-Cell Debate: Ethical Questions
    Stem-Cell Ethics: A Theological Brief

    Stem-Cell Questions
    Theistic Evolution: A Christian Alternative to Atheism, Creationism, and Intelligent Design...
    Theology and Science: Current Issues and Future Directions
    Unscientific America: How science illiteracy threatens our future
    Will ET End Religion?

    Current Stats: topics: >2600, links: >300,000, video: 200 hours.